Catalase Test

Written Questions

1. …

2. Enzyme produced by aerobes and facultative anaerobes

3. Is Streptococcus pyogenes catalase positive or negative?

4. The catalase test, blood hemolysis, and Mannitol fermentation is all used to test what type of

bacteria?

5. This media contains lactose and pH indicators

6. What do MacConkey plates have?

7. If the bacteria produces catalase, you see _______

Multiple Choice Questions

1. …

a. Lactose and pH indicators

b. Gram-positive pathogens

c. Facultative anaerobes

d. The catalase test is used to differentiate between what types of bacteria?

A) Gram positive bacteria

B) Gram negative bacteria

C) enteric pathogens

D) fungal pathogens

2. …

a. Facultative anaerobes

b. The blood hemolysis test is often used to differentiate

A) enteric pathogens

B) Gram positive cocci

C) Gram negative bacteria

D) halophiles

c. Gram-positive pathogens

d. Lactose and pH indicators

3. Is Staphylococcus aureus catalase positive or negative?

a. Negative

b. Gram-positive

c. Catalase

d. Positive

4. What MOs does the catalase help differentiate between?

a. Facultative anaerobes

b. Gram-positive

c. Gram-positive pathogens

d. Positive

5. MOs that grow with or without oxygen, but prefer oxygen

a. Catalase

b. MacConky agar

c. Facultative anaerobes

d. Gram-positive pathogens

6. …

a. Lactose and pH indicators

b. Facultative anaerobes

c. Gram-positive pathogens

d. Mannitol salt agar will select for the growth of what type of organisms?

A) capnophiles

B) anerobes

C) halophiles

D) Gram negative bacteria

True/False Questions

1. MacConkey agar selects for the growth of what type of bacteria?

A) Acid-fast bacteria

B) Gram negative bacteria

C) Gram positive bacteria

D) Endospore-forming bacteria → What do MacConkey plates have?

True False

2. Fermenters on MacConkey agar will appear what color?

A) white

B) colorless

C) yellow

D) red/pink → …

True False

3. What is the carbohydrate source in MacConkey agar?

A) glucose

B) glycerol

C) lactose

D) red blood cells → What do MacConkey plates have?

True False

4. Mannitol salt agar is most useful for differentiating what type of bacteria?

A) Streptococci

B) Staphylococci

C) E. coli strains

D) Bacillus → …

True False

5. MacConkey agar is used for the differentiation of what type of pathogens?

A) upper respiratory pathogens

B) antibiotic resistant pathogens

C) enteric pathogens

D) fungal pathogens → …

True False

6. How can you tell mannitol has been fermented by colonies on mannitol salt agar plates?

A) colonies appear red

B) colonies appear white

C) clear halo forms around the colonies

D) colonies appear yellow → …